Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Control of directionality in lambda site specific recombination.

W Bushman, J F Thompson, L Vargas

    Science (New York, N.Y.)
    |November 22, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    The advantage of using a synoptic pathology report format for cutaneous melanoma.

    Histopathology·2008
    Same author

    Re: NM patients and SN.

    The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·2007
    Same author

    The symptoms of varicose veins: difficult to determine and difficult to study.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2007
    Same author

    Detection of second primary cutaneous melanomas.

    European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2007
    Same author

    Prognostic value of sentinel lymph node biopsy.

    The British journal of dermatology·2007
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    Site-specific recombination directionality in bacteriophage lambda is controlled by protein binding sites and influenced by the Escherichia coli protein integration host factor (IHF). Cellular physiology, specifically growth phase-dependent IHF levels, regulates this crucial DNA transaction.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Site-specific recombination involves DNA transactions with questions about directionality control.
    • Bacteriophage lambda utilizes discrete pathways for viral integration and excision.
    • DNA substrates can be engineered for unidirectional recombination.

    Purpose of the Study:

    • To investigate the control of directionality in bacteriophage lambda site-specific recombination.
    • To understand the role of protein binding sites and cellular factors in recombination directionality.
    • To elucidate the influence of the Escherichia coli integration host factor (IHF) on recombination pathways.

    Main Methods:

    • Analysis of bacteriophage lambda integration and excision pathways.
    • Construction of DNA substrates with intrinsic unidirectional recombination properties.

    Related Experiment Videos

  • Investigation of protein binding site asymmetry and concentration-dependent protein effects.
  • Assessment of the impact of Escherichia coli integration host factor (IHF) on recombination directionality.

    • Main Results:

    • Bacteriophage lambda recombination pathways exhibit asymmetric reliance on protein binding sites.
    • Changes in protein concentrations differentially affect integrative and excisive recombination.
    • The Escherichia coli protein integration host factor (IHF) differentially modulates integrative and excisive recombination, influencing directionality.
    • Intracellular IHF levels increase during the transition from exponential to stationary phase.

    Conclusions:

    • Cellular physiology, specifically growth phase-dependent regulation of IHF, is integral to controlling bacteriophage lambda recombination directionality.
    • The integration host factor (IHF) plays a key role in mediating the directionality of viral DNA recombination.
    • Understanding these regulatory mechanisms provides insights into DNA transaction control within host cellular environments.