Related Experiment Video
Updated: Feb 15, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
Published on: November 1, 2017
Single-shot 3D topography of reflective samples with digital holographic microscopy
Abstract:
In this work, an off-axis digital holographic microscope operating in reflection mode and a telecentric regimen to produce 3D topography of a microscopy sample is shown. The main characteristics of the proposed method, which make it different from the previous works in the field, are the possibility of producing the 3D topography by a single shot over the complete field of view with sensitivity of λ/100, without phase perturbations introduced by the illuminating-imaging system, and with no further numerical processing beyond that required for recovering the phase map of the sample. A complete analysis of the illuminating-imaging system of the digital holographic microscope is presented. The proposed digital holographic microscope is tested on imaging a USAF resolution test target and some micro-electromechanical systems (MEMs).
Related Concept Videos
Total Internal Reflection Fluorescence Microscopy
Methods of Obtaining Topography
Preparation of Samples for Electron Microscopy
Reflection of Waves
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
The Sense of Self: Reflected Self-Appraisal and Social Comparison

