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Updated: Feb 15, 2026

Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
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pDok2, caspase 3 dependent glioma cell growth arrest by nitidine chloride

Ravindra Pramod Deshpande1, Phanithi Prakash Babu1

  • 1Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana State, India.

Pharmacological Reports : PR
|January 13, 2018
PubMed
Abstract

Insights

Nitidine chloride (NC) shows potential as an anti-glioma drug by inhibiting pDok2 and inducing cell death in glioblastoma cells. Further research is needed to explore its mechanisms against brain tumors.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Nitidine chloride (NC) exhibits anticancer and anti-metastatic properties across various tumors.
  • NC has demonstrated inhibition of the PI3K/AKT/mTOR pathway in U87 human glioma cells.

Purpose of the Study:

  • To investigate the anti-glioma effects of Nitidine chloride (NC) in C6 rat glioma and U87 human glioblastoma cells.
  • To elucidate the molecular mechanisms underlying NC's action, focusing on pDok2 and caspase-3 dependent cell death.

Main Methods:

  • Cell viability, proliferation, and migration were assessed using MTT, clonogenic, and wound healing assays.
  • Cell cycle progression was analyzed via flow cytometry (FACS).
  • Western blotting was employed to measure protein levels of pDok2, caspase-3, PARP, and Gsk3 beta.

Main Results:

  • NC inhibited pDok2 expression in U87 cells in a dose-dependent manner.
  • Increased cleaved PARP and cleaved caspase-3 were observed in NC-treated C6 cells.
  • NC demonstrated significant inhibition of C6 cell growth and colony formation at 8μM, inducing G2/M phase cell cycle arrest and inhibiting Gsk3 beta.

Conclusions:

  • Nitidine chloride (NC) effectively inhibits glioblastoma cell growth and induces apoptosis.
  • NC targets key proteins including pDok2 and Gsk3 beta, suggesting a multifaceted anti-glioma mechanism.
  • NC warrants further investigation as a potential therapeutic agent for glioma.
Keywords:
CaspaseGliomaGsk3 betaNitidine chlorideWound healing

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