Meat species identification using DNA-luminol interaction and their slow diffusion onto the biochip surface

Area of Science:

• Food Science
• Analytical Chemistry
• Biotechnology

Background:

• Consumer concerns about food authenticity and adulteration, particularly with porcine DNA, are increasing.
• Accurate detection methods are crucial for verifying food ingredient integrity and preventing fraud.

Purpose of the Study:

• To develop a novel, simple, sensitive, and rapid luminol-based electrochemiluminescence (ECL) technique for detecting Sus scrofa (porcine) DNA.
• To create a potential biosensor for verifying food authenticity.

Main Methods:

• Porcine DNA amplification using loop-mediated isothermal amplification (LAMP).
• Quantification of amplified DNA via luminol-based electrochemiluminescence (ECL).
• Utilizing carbon screen-printed electrodes (SPE) for the ECL analysis.

Main Results:

• The developed LAMP-ECL sensor demonstrated high specificity and sensitivity (≥0.1 pg/μL).
• The detection process was rapid, completing in approximately 5 minutes.
• Formation of DNA-luminol complexes hindered luminol diffusion, leading to measurable changes in light intensity.

Conclusions:

• The novel LAMP-ECL technique offers a reliable method for porcine DNA detection.
• This approach has significant potential for development into a compact biosensor for food authenticity verification.
• The method addresses the need for rapid and sensitive detection of food adulteration.