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Hyaluronidase and retinal function.

B S Winkler, E M Cohn

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |November 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Hyaluronidase, an enzyme, did not affect electroretinograms (ERG) or metabolic activity in isolated rat retinas. This suggests hyaluronidase is safe for ocular irrigating solutions and potentially useful for enzyme-assisted vitrectomy.

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    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Enzymology

    Background:

    • Hyaluronidase is an enzyme known to degrade hyaluronic acid.
    • Its potential use in ocular surgery, specifically vitrectomy, requires evaluation of its effects on retinal function and metabolism.

    Purpose of the Study:

    • To investigate the effects of hyaluronidase on the electroretinogram (ERG) and metabolic activities of isolated rat retinas.
    • To determine the safety and potential utility of hyaluronidase in ocular irrigating solutions.

    Main Methods:

    • Isolated rat retinas were superfused with a buffered, oxygenated medium containing varying concentrations of hyaluronidase (100–5,000 units/mL).
    • Electroretinograms (ERG) were recorded to assess retinal function (a and b waves).
    • Metabolic activities, including lactic acid production, oxygen consumption, glutathione content, and ATPase activity, were measured.

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    Main Results:

    • Hyaluronidase concentrations did not significantly alter the amplitudes of ERG a waves and b waves compared to controls.
    • Metabolic parameters (lactic acid, oxygen consumption, glutathione, ATPase activity) remained similar in hyaluronidase-exposed retinas and control retinas.
    • The enzyme effectively liquefied postmortem human vitreous at 1,000 units/mL within 15-30 minutes.

    Conclusions:

    • Hyaluronidase, at concentrations tested, does not adversely affect ERG or retinal metabolic activity in isolated rat retinas.
    • These findings support the safety of hyaluronidase in ocular irrigating solutions.
    • Hyaluronidase shows promise for use in enzyme-assisted vitrectomy procedures.