Related Experiment Video
Updated: Feb 15, 2026
09:13
Method to Obtain Pattern of Breathing in Senescent Mice through Unrestrained Barometric Plethysmography
Published on: April 28, 2020
7.1K
Testing Acute Oxygen Sensing in Genetically Modified Mice: Plethysmography and Amperometry
Patricia Ortega-Sáenz1,2,3, Candela Caballero4,5,6,7, Lin Gao4,5,6
1Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain. gortega1@us.es.
Methods in Molecular Biology (Clifton, N.J.)
|January 14, 2018
Summary
Investigating oxygen sensing mechanisms requires monitoring animal and cell responses to low oxygen. This study details methods for assessing ventilatory responses and catecholamine release, crucial for understanding oxygen homeostasis.
Area of Science:
- Physiology
- Cell Biology
- Neuroscience
Background:
- Oxygen sensing is vital for physiological homeostasis.
- Understanding oxygen sensing mechanisms requires robust experimental protocols.
- Chemoreceptor cells in the carotid body and adrenal medulla are key players in oxygen sensing.
Purpose of the Study:
- To describe laboratory protocols for evaluating the physiological response to acute hypoxia.
- To detail methods for monitoring single-cell catecholamine release from oxygen-sensing cells.
- To provide a framework for studying oxygen sensing in normal and genetically modified models.
Main Methods:
- Evaluation of ventilatory response to acute hypoxia in whole animals (normal and genetically modified).
- Amperometric technique for real-time monitoring of single-cell catecholamine release.
- Preparation of carotid body and adrenal medulla slices for cellular analysis.
Main Results:
- Established protocols allow for comprehensive assessment of hypoxic responses.
- Demonstrated feasibility of monitoring catecholamine release from specific cell types.
- Provided reproducible methods applicable to diverse research questions in oxygen sensing.
Conclusions:
- The described protocols are essential for dissecting oxygen sensing pathways.
- These methods facilitate the investigation of cellular and systemic responses to hypoxia.
- This work supports further research into oxygen homeostasis and related disorders.
Related Concept Videos
01:10Amperometry: Overview
1.8K
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
1.8K
02:57The Sense of Self: Reflected Self-Appraisal and Social Comparison
56.2K
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
56.2K
02:59Animal Mitochondrial Genetics
9.3K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.3K
01:26Introduction to Special Senses
7.7K
Sensory receptors play an integral part in comprehending our external and internal environments. They receive diverse stimuli, converting them into the nervous system's electrochemical signals. This conversion occurs as the stimulus alters the sensory neuron's cell membrane potential, instigating the generation of an action potential. This action potential is subsequently transmitted to the central nervous system (CNS), which integrates with other sensory data or higher cognitive...
7.7K
02:16Genetics of Speciation
22.0K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
22.0K
01:25What is Population Genetics?
65.0K
A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
65.0K