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Published on: May 21, 2015
Detection of Hypoxia and HIF in Paraffin-Embedded Tumor Tissues
Fuming Li1,2, Kyoung Eun Lee1,2, M Celeste Simon3,4
1Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA, USA.
Abstract:
Hypoxia (insufficient O2 availability) is involved in various biological processes, such as tumorigenesis and inflammation. Hypoxia results in stabilization of hypoxia-inducible factors (HIFs) including HIF1α and HIF2α. Here we describe a protocol to detect mouse and human tissue hypoxia by using Hypoxyprobe and immunohistochemical staining for HIF1α and HIF2α.
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