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Determination of meropenem in endotracheal tubes by in-tube solid phase microextraction coupled to capillary liquid

L Hakobyan1, J Pla Tolos1, Y Moliner-Martinez1

  • 1Department of Analytical Chemistry, University of Valencia, Dr Moliner, 50, 46100, Burjassot, Spain.

Journal of Pharmaceutical and Biomedical Analysis
|January 15, 2018
PubMed

Insights

This study developed a method to measure meropenem in endotracheal tubes (ETTs) to assess its biofilm penetration. The new technique successfully quantified meropenem in patient ETTs, aiding antimicrobial treatment effectiveness evaluation.

Area of Science:

  • Analytical Chemistry
  • Pharmacokinetics
  • Microbiology

Background:

  • Meropenem is crucial for treating intensive care unit (ICU) infections linked to medical devices.
  • Antimicrobial efficacy is often limited by poor penetration into biofilms.
  • Analyzing drug concentrations in endotracheal tubes (ETTs) is challenging but vital for in-vivo studies.

Purpose of the Study:

  • To develop and validate an in-tube solid phase microextraction (SPME) coupled with capillary liquid chromatography (CapLC) and diode array detection (DAD) method.
  • To quantify meropenem concentrations within ETTs to evaluate its penetration into biofilms.
  • To establish an effective extraction method for meropenem from ETT samples.

Main Methods:

  • Optimization of in-tube SPME parameters including sample volume, capillary length, flow rate, and coating.
  • Development of CapLC-DAD conditions using a gradient mobile phase of acetonitrile-water.
  • Evaluation of different extraction techniques: liquid extraction, vortex-assisted liquid extraction (VALE), and ultrasound-extraction (UAE).

Main Results:

  • Optimized in-tube SPME involved processing 500 μL of sample at 9 μL/seg using a 60-cm capillary column (35% diphenyl/65% polydimethylsiloxane).
  • The validated in-tube SPME-CapLC-DAD method demonstrated good sensitivity (LOD = 3 μg/L) and precision (RSD < 10%).
  • Ultrasound-assisted extraction achieved quantitative meropenem recovery (91 ± 6%) from ETTs within 1 minute using water as the solvent.

Conclusions:

  • A robust in-tube SPME-CapLC-DAD method was successfully developed for meropenem determination in ETTs.
  • The method allows for the assessment of meropenem penetration into biofilms in patient-derived ETTs.
  • This approach provides valuable insights into antimicrobial efficacy in complex clinical settings.
Keywords:
BiofilmCapillary-LC-DADEndotracheal tubesIn-tube SPMEMeropenem

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