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Updated: Feb 15, 2026

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
Published on: October 11, 2012
Markers of Developmentally Regulated Programmed Cell Death and Their Analysis in Cereal Seeds
Fernando Domínguez1, Francisco Javier Cejudo2
1Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla and CSIC, Sevilla, Spain.
Abstract:
Programmed cell death (PCD) is a key process for the development and differentiation of multicellular organisms, which is characterized by well-defined morphological and biochemical features. These include chromatin condensation, DNA degradation and nuclear fragmentation, with nucleases and proteases playing a relevant function in these processes. In this chapter we describe methods routinely used for the analysis of hallmarks of developmentally regulated PCD in cereal seed tissues, which are based on agarose and polyacrylamide gel electrophoresis, in situ staining of DNA fragmentation, and cell-free assays of relevant enzymatic activities.
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