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Related Experiment Video

Updated: Feb 15, 2026

Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria

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Published on: May 16, 2025

Plant Cell Cultures as Model Systems to Study Programmed Cell Death.

Sara Cimini¹, Maria Beatrice Ronci¹, Elisabetta Barizza²

¹Food Sciences and Human Nutrition Unit, University Campus Bio-Medico of Rome, Rome, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|January 15, 2018

Summary

Investigating programmed cell death (PCD) in plants is complex. This study presents methods for plant cell cultures to quantify cell viability and analyze PCD markers, aiding research into plant signaling pathways.

Keywords:

Cell culture Cell viability/death analysis Morphological and genetic markers Real-time polymerase chain reaction

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Published on: November 10, 2023

Area of Science:

Plant biology
Cellular biology
Molecular biology

Background:

Studying programmed cell death (PCD) in whole plants presents significant challenges.
Plant cell suspension cultures offer a homogeneous system for investigating PCD triggered by environmental and developmental stimuli.
Quantifying cell viability and death over time is a primary hurdle in analyzing PCD within cell cultures.

Purpose of the Study:

To establish and propagate diverse plant cell cultures, including crop species.
To develop accurate methods for determining cell viability and identifying PCD morphological and genetic markers.
To optimize nucleic acid extraction protocols for real-time PCR analysis in challenging cell culture systems.

Main Methods:

Establishment and propagation of plant cell suspension cultures from various species.
Development of adapted methods for cell viability assessment in plant cell cultures.
Optimization of nucleic acid extraction for real-time PCR, particularly for cultures unsuitable for commercial kits.

Main Results:

Accurate methods for establishing and maintaining plant cell cultures were detailed.
Protocols for assessing cell viability and identifying PCD markers were established.
An optimized nucleic acid extraction protocol was developed for real-time PCR in specific cell cultures.

Conclusions:

Plant cell cultures provide a valuable model for studying programmed cell death (PCD).
The developed methods facilitate accurate quantification of cell viability and analysis of PCD markers.
Optimized protocols enhance the study of PCD signaling pathways using molecular techniques like real-time PCR.