Advances and current challenges in calcium signaling

Content Summary 414 I. Introduction 415 II. Ca²⁺ importer and exporter in plants 415 III. The Ca²⁺ decoding toolkit in plants 415 IV. Mechanisms of Ca²⁺ signal decoding 417 V. Immediate Ca²⁺ signaling in the regulation of ion transport 418 VI. Ca²⁺ signal integration into long-term ABA responses 419 VII Integration of Ca²⁺ and hormone signaling through dynamic complex modulation of the CCaMK/CYCLOPS complex 420 VIII Ca²⁺ signaling in mitochondria and chloroplasts 422 IX A view beyond recent advances in Ca²⁺ imaging 423 X Modeling approaches in Ca²⁺ signaling 424 XI Conclusions: Ca²⁺ signaling a still young blooming field of plant research 424 Acknowledgements 425 ORCID 425 References 425 SUMMARY: Temporally and spatially defined changes in Ca²⁺ concentration in distinct compartments of cells represent a universal information code in plants. Recently, it has become evident that Ca²⁺ signals not only govern intracellular regulation but also appear to contribute to long distance or even organismic signal propagation and physiological response regulation. Ca²⁺ signals are shaped by an intimate interplay of channels and transporters, and during past years important contributing individual components have been identified and characterized. Ca²⁺ signals are translated by an elaborate toolkit of Ca²⁺ -binding proteins, many of which function as Ca²⁺ sensors, into defined downstream responses. Intriguing progress has been achieved in identifying specific modules that interconnect Ca²⁺ decoding proteins and protein kinases with downstream target effectors, and in characterizing molecular details of these processes. In this review, we reflect on recent major advances in our understanding of Ca²⁺ signaling and cover emerging concepts and existing open questions that should be informative also for scientists that are currently entering this field of ever-increasing breath and impact.