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Updated: Feb 15, 2026
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Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
Published on: May 10, 2020
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When Long Noncoding RNAs Meet Genome Editing in Pluripotent Stem Cells.
Fuquan Chen1,2, Jiaojiao Ji1,2, Jian Shen1,2
1State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300371, China.
Stem Cells International
|January 16, 2018
Summary
Genome editing technologies are advancing the study of long noncoding RNAs (lncRNAs) in pluripotent stem cells (PSCs). This research highlights tools for dissecting lncRNA functions in these crucial cells.
Area of Science:
- Genomics
- Molecular Biology
- Stem Cell Biology
Background:
- The human genome extensively transcribes RNA, with most regions producing noncoding RNAs rather than protein-coding mRNAs.
- Long noncoding RNAs (lncRNAs) play critical regulatory roles in biological processes like imprinting and transcriptional regulation.
- While protein-coding genes are well-studied using genome editing in pluripotent stem cells (PSCs), lncRNA research with these methods is emerging.
Purpose of the Study:
- To summarize recent progress in using genome editing technologies to study lncRNA functions in PSCs.
- To identify and highlight potential genome editing tools applicable for lncRNA research in PSCs.
Main Methods:
- Review of recent advancements in genome editing technologies applied to lncRNA research.
- Identification of specific genome editing tools suitable for functional studies of lncRNAs in PSCs.
Main Results:
- Genome editing is increasingly utilized to investigate the roles of lncRNAs in PSCs.
- Various genome editing tools show promise for dissecting lncRNA functions in the context of pluripotency.
Conclusions:
- Genome editing technologies offer powerful approaches to elucidate the functions of lncRNAs in PSCs.
- Further application of these tools will enhance our understanding of lncRNA-mediated biological regulation in stem cells.
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