Related Experiment Video

Updated: Feb 15, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.7K

The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5

Abdullah Shah1, Farooq Rashid1, Hassaan Mehboob Awan1

  • 1CAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, China.

Stem Cells International
|January 16, 2018
PubMed

Insights

DEAD-box RNA helicase DDX3 interacts with ALKBH5, a key enzyme in RNA modification. This study reveals DDX3

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Biochemistry

Background:

  • DEAD-box RNA helicases, including DDX3, are crucial for various cellular processes.
  • m6A RNA modification is a dynamic regulatory mechanism in gene expression.
  • ALKBH5 is a known m6A RNA demethylase involved in RNA metabolism.

Purpose of the Study:

  • To investigate the interaction between DDX3 and ALKBH5.
  • To elucidate the role of DDX3 in regulating m6A RNA demethylation.
  • To explore the impact of DDX3 on microRNA methylation.

Main Methods:

  • Co-immunoprecipitation assays to confirm protein-protein interactions.
  • In vitro assays to assess the functional impact of DDX3 on ALKBH5 activity.
  • Analysis of microRNA methylation status in cells expressing varying levels of DDX3.

Main Results:

  • DDX3 directly interacts with ALKBH5, with specific domains (ATP for DDX3, DSBH for ALKBH5) being essential for this interaction.
  • DDX3 modulates the demethylation activity of ALKBH5 on mRNAs.
  • DDX3 influences the methylation status of microRNAs and interacts with AGO2.

Conclusions:

  • DDX3 plays a regulatory role in m6A RNA demethylation.
  • The interaction between DDX3 and ALKBH5 provides a new link in understanding RNA modification dynamics.
  • DDX3's involvement extends to microRNA regulation, highlighting its multifaceted role in RNA metabolism.

More Related Videos

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.9K
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

11.3K

Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.2K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.3K
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.2K