Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles
Qihui Fan1,2, Wenqi Hu2, Aaron T Ohta2
1College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Abstract:
Localized single cells can be lysed precisely and selectively using microbubbles optothermally generated by microsecond laser pulses. The shear stress from the microstreaming surrounding laser-induced microbubbles and direct contact with the surface of expanding bubbles cause the rupture of targeted cell membranes. High-resolution single-cell lysis is demonstrated: cells adjacent to targeted cells are not lysed. It is also shown that only a portion of the cell membrane can be punctured using this method. Both suspension and adherent cell types can be lysed in this system, and cell manipulation can be integrated for cell-cell interaction studies.
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