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Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles.

Qihui Fan1,2, Wenqi Hu2, Aaron T Ohta2

  • 1College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

Micromachines
|January 16, 2018
PubMed
Summary
This summary is machine-generated.

Precise single-cell lysis is achieved using laser-activated microbubbles. This optothermal method selectively ruptures targeted cell membranes with minimal damage to adjacent cells, enabling new research possibilities.

Keywords:
cell lysiscell manipulationcell porationoptothermal

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Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Engineering

Background:

  • Precise manipulation and lysis of individual cells are crucial for various biological studies.
  • Existing cell lysis methods often lack selectivity or can damage surrounding cells.

Purpose of the Study:

  • To develop and demonstrate a highly selective and precise method for single-cell lysis using optothermally generated microbubbles.
  • To investigate the mechanisms of cell membrane rupture induced by laser-generated microbubbles.

Main Methods:

  • Microbubbles were generated optothermally using microsecond laser pulses.
  • Shear stress from microstreaming and bubble expansion were utilized to lyse targeted cells.
  • High-resolution imaging was employed to verify lysis selectivity and membrane integrity.

Main Results:

  • Demonstrated high-resolution single-cell lysis with no lysis of adjacent cells.
  • Showed that only a portion of the cell membrane can be punctured.
  • Successfully lysed both suspension and adherent cell types.
  • Integrated cell manipulation capabilities for cell-cell interaction studies.

Conclusions:

  • Optothermal microbubble generation provides a precise and selective method for single-cell lysis.
  • The technique offers control over the extent of cell membrane damage.
  • This method is applicable to diverse cell types and can be combined with cell manipulation for advanced research.