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Epigenetic inactivation of RUNX3 in colorectal cancer

Eung Jin Shin1, Han Jo Kim2, Myoung Won Son1

  • 1Department of Surgery, Soonchunhyang University College of Medicine, Cheonan, Korea.

Annals of Surgical Treatment and Research
|January 16, 2018
PubMed
Abstract

Insights

RUNX3 hypermethylation is linked to poor prognosis in colorectal cancer (CRC). This epigenetic change, not RUNX3 expression, may predict outcomes in CRC patients, highlighting its clinical significance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Runt-related transcription factor 3 (RUNX3) acts as a tumor suppressor gene in various cancers.
  • The clinical impact of RUNX3 inactivation in colorectal cancer (CRC) requires further elucidation.

Purpose of the Study:

  • To investigate the association between clinicopathological factors and RUNX3 promoter hypermethylation and RUNX3 gene expression in CRC patients.

Main Methods:

  • Analyzed 62 CRC tissue samples using methylation-specific PCR for RUNX3 promoter hypermethylation.
  • Assessed RUNX3 mRNA expression via reverse transcriptase-PCR and protein levels through immunohistochemical staining.

Main Results:

  • RUNX3 promoter hypermethylation was observed in 32.3% of CRC samples.
  • Hypermethylation correlated significantly with vascular invasion (P=0.006) and lymphatic invasion (P=0.002).
  • RUNX3 hypermethylation was associated with poorer survival outcomes (P=0.038), while RUNX3 expression was not a prognostic factor (P=0.363).

Conclusions:

  • RUNX3 promoter hypermethylation may serve as a predictive biomarker for poor prognosis in colorectal cancer.
Keywords:
Colorectal neoplasmsImmunohistochemistryMethylationPrognosisRUNX3 protein

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