Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oral Cavity01:11

Oral Cavity

3.2K
The oral cavity, or the mouth, is a complex structure in humans that plays a vital role in our day-to-day lives. Its role is not only in chewing and swallowing food; it also plays a role in speech and facial expressions.
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
3.2K
Oral Hypoglycemic Agents: Glinides01:06

Oral Hypoglycemic Agents: Glinides

700
Repaglinide (Prandin) and Nateglinide (Starlix), known as glinides, are oral insulin secretagogues that stimulate insulin release from pancreatic β cells by closing the ATP-sensitive potassium channels (KATP channel). Repaglinide controls insulin release from pancreatic β cells by managing potassium efflux. It shares two binding sites with sulfonylureas and also has a unique site, indicating overlapping mechanisms of action. With a rapid onset and a 4-7 hour duration, it effectively...
700
Psychosexual Stages of Personality: Oral01:16

Psychosexual Stages of Personality: Oral

3.3K
The oral stage is the initial phase of Sigmund Freud's theory of psychosexual development, occurring from birth to approximately 12 to 18 months. During this period, the infant's mouth serves as the primary source of pleasure, with actions such as sucking, chewing, biting, and drinking playing a crucial role in reducing tension. These activities are essential not only for nourishment but also for the infant's psychological and emotional satisfaction.
Weaning, typically occurring...
3.3K
Oral Hypoglycemic Agents: Sulfonylureas01:17

Oral Hypoglycemic Agents: Sulfonylureas

1.2K
Sulfonylureas are oral hypoglycemic agents utilized in treating type 2 diabetes. They are characterized by their unique sulfonylurea chemical structure. The family of sulfonylureas is divided into generations. First-generation sulfonylureas, including tolbutamide (Orinase), chlorpropamide (Diabinese), and tolazamide (Tolinase), trigger insulin release from pancreatic β cells and enhance peripheral tissues' insulin sensitivity. The second-generation members, such as glipizide...
1.2K
Assessing Body Temperature - Oral01:14

Assessing Body Temperature - Oral

1.7K
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
1.7K
Non-Oral Extravascular Drug Absorption Routes01:15

Non-Oral Extravascular Drug Absorption Routes

551
Non-oral extravascular routes, which encompass sublingual, buccal, topical, intramuscular, and inhalation methods, primarily utilize passive diffusion to transport drugs into the systemic circulation. The absorption rates and effectiveness of these routes depend on the drug's physicochemical properties, as well as the patient's anatomical and pathophysiological state.
Lipophilic drugs that are stable at salivary pH (6) and exhibit minimal binding to the oral mucosa are absorbed more...
551

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Specific Deletion of Interleukin-1 Beta in Microglia Improves Acute Outcome and Modulates Neurogenesis After Ischemic Stroke.

Glia·2026
Same author

Bone marrow B cell collapse promotes bone metastasis in breast cancer.

bioRxiv : the preprint server for biology·2026
Same author

Clinical features, genetics, treatment, and long-term outcomes of STAT3 hyper-IgE syndrome: Single-center cohort analysis.

The Journal of allergy and clinical immunology·2026
Same author

Mucosal Inflammation Shapes Human Neutrophil States in Tissue and Circulation.

bioRxiv : the preprint server for biology·2026
Same author

Ketogenic diet synergistic reprogramming of both host and microbiome promotes tissue regeneration.

bioRxiv : the preprint server for biology·2026
Same author

Oral lichenoid lesions in CTLA-4 haploinsufficiency: Targeting IFN-γ-driven mucosal immunopathology.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026
Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026
Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026
Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026
Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026
Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026
See all related articles

Related Experiment Video

Updated: Feb 15, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.9K

Oral Microbiome Characterization in Murine Models.

Loreto Abusleme1, Bo-Young Hong2, Anilei Hoare2

  • 1Oral Immunity and Inflammation Unit, NIDCR, NIH, Bethesda, MD, USA.

Bio-Protocol
|January 16, 2018
PubMed
Summary
This summary is machine-generated.

This study details a method for analyzing the oral microbiome in mice. It covers sample collection, processing, and characterization using 16S rRNA sequencing for immune studies.

Keywords:
16S rRNA gene sequencingGingival microbiomeMicrobiome sequencingMouse oral samplingMurine oral microbiotaOral microbiome

More Related Videos

Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis
06:39

Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis

Published on: October 27, 2023

1.5K
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

12.4K

Related Experiment Videos

Last Updated: Feb 15, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.9K
Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis
06:39

Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis

Published on: October 27, 2023

1.5K
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

12.4K

Area of Science:

  • Microbiology
  • Immunology
  • Genomics

Background:

  • The oral microbiome plays a crucial role in oral immunity and is linked to inflammatory conditions like periodontitis.
  • Understanding the oral microbiome's composition is vital for studying host-microbe interactions.
  • Murine models are essential for investigating oral health and disease mechanisms.

Purpose of the Study:

  • To establish a standardized protocol for oral microbiome characterization in murine models.
  • To enable the study of oral microbiome dynamics under various physiological and immunological conditions.
  • To provide a foundation for research into periodontitis and other oral inflammatory diseases.

Main Methods:

  • Detailed procedures for oral microbiome sampling in mice.
  • Methods for processing low-biomass samples to maximize DNA yield.
  • 16S rRNA gene sequencing for microbial community profiling.

Main Results:

  • A reproducible protocol for oral microbiome analysis in mice was developed.
  • The protocol allows for characterization of oral microbial communities at steady state and during perturbations.
  • Successful application of 16S rRNA sequencing to low-biomass oral samples.

Conclusions:

  • This protocol facilitates comprehensive oral microbiome analysis in murine models.
  • It supports research into the role of the oral microbiome in health and disease.
  • The method is applicable to studies involving immune or physiological changes affecting the oral cavity.