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Updated: Feb 15, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Oral Microbiome Characterization in Murine Models
Loreto Abusleme1, Bo-Young Hong2, Anilei Hoare2
1Oral Immunity and Inflammation Unit, NIDCR, NIH, Bethesda, MD, USA.
Abstract:
The oral microbiome has been implicated as a trigger for immune responsiveness in the oral cavity, particularly in the setting of the inflammatory disease periodontitis. The protocol presented here is aimed at characterizing the oral microbiome in murine models at steady state and during perturbations of immunity or physiology. Herein, we describe murine oral microbiome sampling procedures, processing of low biomass samples and subsequent microbiome characterization based on 16S rRNA gene sequencing.
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