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Related Experiment Videos

Possible pathways for lysosomal enzyme delivery.

H J Geuze, J W Slot, G J Strous

    The Journal of Cell Biology
    |December 1, 1985
    PubMed
    Summary

    This study reveals that mannose 6-phosphate receptor (MPR) and lysosomal enzymes use coated vesicles from the trans-Golgi reticulum (TGR) for secretion in Hep G2 cells. This pathway also transports albumin, suggesting a novel secretory route.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • The intracellular trafficking of proteins, particularly lysosomal enzymes and receptors, is crucial for cellular function.
    • Understanding the specific pathways involved in protein sorting and secretion is essential for deciphering cellular processes.

    Purpose of the Study:

    • To investigate the subcellular distribution of mannose 6-phosphate receptor (MPR), lysosomal enzymes, and albumin in Hep G2 cells.
    • To elucidate the role of the trans-Golgi reticulum (TGR) and CURL (compartment of uncoupling receptors and ligands) in protein trafficking.
    • To determine the involvement of coated vesicles in the secretory pathway.

    Main Methods:

    • Immunogold double-labeling and ultrathin cryosections were employed.
    • Hep G2 cells were analyzed for the localization of MPR, galactosyltransferase, lysosomal enzymes (cathepsin D, beta-hexosaminidase, alpha-glucosidase), and albumin.
    • Cells were treated with primaquine to observe effects on MPR distribution.

    Main Results:

    • MPR and lysosomal enzymes were found throughout the Golgi and in TGR, with MPR also abundant in CURL.
    • Albumin was detected in TGR and coated buds originating from TGR.
    • Primaquine treatment led to MPR accumulation in TGR and coated buds, suggesting a role in secretion.

    Conclusions:

    • Coated vesicles originating from TGR play a role in the secretory pathway for albumin, MPR, and lysosomal enzymes in Hep G2 cells.
    • CURL appears to be involved in the transport of lysosomal enzymes to lysosomes.
    • The MPR system utilizes this TGR-derived secretory route.

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