Related Experiment Video
Updated: Feb 15, 2026
11:55
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Published on: August 16, 2016
12.2K
Highly parallel direct RNA sequencing on an array of nanopores
Daniel R Garalde1, Elizabeth A Snell1, Daniel Jachimowicz1
1Oxford Nanopore Technologies Ltd., Oxford, UK.
Nature Methods
|January 16, 2018
Summary
Nanopore direct RNA sequencing offers full-length, strand-specific RNA reads without reverse transcription or amplification. This single-molecule method overcomes limitations of current RNA sequencing techniques.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- RNA sequencing provides insights into biological processes but faces limitations.
- Current RNA sequencing methods suffer from short read lengths and biases from reverse transcription or amplification.
Purpose of the Study:
- To introduce a novel nanopore direct RNA sequencing method.
- To overcome the limitations of existing RNA sequencing technologies.
Main Methods:
- Utilized nanopore technology for direct RNA sequencing.
- Employed a highly parallel, real-time, single-molecule approach.
- Circumvented the need for reverse transcription or amplification steps.
Main Results:
- Achieved full-length, strand-specific RNA sequencing.
- Enabled direct detection of nucleotide analogs within RNA.
- Demonstrated a method free from reverse transcription or amplification biases.
Conclusions:
- Nanopore direct RNA sequencing is a powerful tool for comprehensive RNA analysis.
- This method enhances the accuracy and scope of RNA sequencing applications.
- Future research can leverage this technique for diverse biological investigations.
Related Concept Videos
01:23Types of RNA
73.2K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.2K
01:23RNA Interference
28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
01:32RNA Splicing
60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
01:20Parallel Processing
780
The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
780
01:23Parallel Resonance
614
The parallel RLC circuit is an arrangement where the resistor (R), inductor (L), and capacitor (C) are all connected to the same nodes and, as a result, share the same voltage across them. The parallel RLC circuit is analyzed in terms of admittance (Y), which reflects the ease with which current can flow. The admittance is given by:
614
01:53RNA Stability
35.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K