Related Experiment Video
Updated: Feb 15, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
Direct Electrochemical Vibrio DNA Sensing Adopting Highly Stable Graphene-Flavin Mononucleotide Aqueous Dispersion
Tao Yang1, Huaiyin Chen1,2, Zhiwei Qiu1
1Key Laboratory of Sensor Analysis of Tumor Marker of Education Ministry, Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology , Qingdao 266042, P. R. China.
Abstract:
A biofunctionalized graphene nanohybrid was prepared by simultaneously sonicating graphene and riboflavin 5'-monophosphate sodium salt (FMNS). FMNS, as a biodispersant, showed an efficient stabilization for obtaining highly dispersed graphene nanosheets in an aqueous solution. Due to the superior dispersion of graphene and the excellent electrochemical redox activity of FMNS, a direct electrochemical DNA sensor was fabricated by adopting the inherent electrochemical redox activity of graphene-FMNS (Gr-FMNS). The comparison between using traditional electrochemical indicator ([Fe(CN)6]3-/4-) and using the self-signal of Gr-FMNS was fully conducted to study the DNA-sensing performance. The results indicate that the proposed DNA-sensing platform displays fine selectivity, high sensitivity, good stability, and reproducibility using either [Fe(CN)6]3-/4- probe or the self-signal of Gr-FMNS. The two methods display the same level of detection limit: 7.4 × 10-17 M (using [Fe(CN)6]3-/4-) and 8.3 × 10-17 M (using self-signal), respectively, and the latter exhibits higher sensitivity. Furthermore, the sensing platform also can be applied for the DNA determination in real samples.
More Related Videos
09:15Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors
Published on: November 22, 2016
08:57Scalable Syntheses of Graphene Oxide and Reduced Graphene Oxide using Cascade Design Oxidation and Highly Basic Reduction Reactions
Published on: July 3, 2025
Related Concept Videos
What is an Electrochemical Gradient?
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
Chemical Reactions in Aqueous Solutions
Distribution and Dispersion
Aqueous Solutions and Heats of Hydration
When ionic compounds dissolve in water, the ions in the solid separate and disperse uniformly throughout the solution because water molecules surround and solvate the ions, reducing the strong electrostatic forces between them. This process...
Protein-protein Interfaces
The Sense of Self: Reflected Self-Appraisal and Social Comparison