Related Experiment Video
Updated: Feb 15, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Genome Evolution Analysis of Recurrent Testicular Malignant Mesothelioma by Whole-Genome Sequencing
Shigeng Zhang1, Qi Zhang2, Qing Sun3
1Department of Surgical Urology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Background/Aims:
Malignant mesothelioma of the tunica vaginalis testis is a rare and lethal disease. The genomic characteristics and genetic changes of tumor cells during the progression of this disease are unknown.
Methods:
we performed whole-genome sequencing of four successive tumor samples derived from surgery and a blood sample in a single patient.
Results:
All tumors were found to have significant C-to-T and T-to-C mutations, and amplification of copy number in chromosomes 1 and 12 were notified in all tumor samples. Subclone analysis revealed a parallel evolution of the tumor in this patient. We also identified some mutations in mesothelioma-associated genes such as KIF25, AHNAK, and PRDM2.
Conclusions:
The results showed a comprehensive genomic change in malignant mesothelioma of the tunica vaginalis testis and provide a better understanding of the clonal evolution during tumor recurrence and metastasis.
Related Concept Videos
Genome Size and the Evolution of New Genes
Genome Size and the Evolution of New Genes
Genomics
Genomic Imprinting and Inheritance
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...

