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Updated: Feb 15, 2026
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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Circular RNA expression in ovarian endometriosis
Licong Shen1, Yu Zhang1, Wenjun Zhou1
1Department of Obstetrics & Gynecology, Xiangya Hospital, Central South University, No 87 Xiangya Road, Changsha Hunan 410008, PR China.
Epigenomics
|January 17, 2018
Summary
Circular RNAs (circRNAs) show altered expression in endometriosis, potentially impacting disease development. These findings highlight circRNAs as key players in endometriosis pathogenesis.
Area of Science:
- Molecular Biology
- Genetics
- Reproductive Biology
Background:
- Circular RNAs (circRNAs) with miRNA response elements (MREs) act as competing RNA (ceRNA) regulators of gene expression.
- Understanding circRNA roles is crucial for endometriosis research.
Purpose of the Study:
- To identify the expression profile of circRNAs in endometriosis.
- To investigate the potential role of circRNAs in endometriosis pathogenesis.
Main Methods:
- Microarray analysis of ovarian endometriomas and eutopic endometrium.
- Quantitative real-time RT-PCR validation in 24 patient samples.
- Bioinformatic prediction of MREs, ceRNA networks, and KEGG pathways.
Main Results:
- Identified 262 upregulated and 291 downregulated circRNAs in endometriosis.
- Predicted 1225 MREs, a ceRNA network of 122 miRNAs and 137 mRNAs.
- Associated these networks with nine biological pathways.
Conclusions:
- CircRNAs are differentially expressed in endometriosis.
- These dysregulated circRNAs may contribute to the pathogenesis of endometriosis.
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