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Related Concept Videos

Adhesion01:14

Adhesion

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Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
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Cell Adhesion in Plants01:14

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Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
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pH Regulation in Cells01:28

pH Regulation in Cells

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pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
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Immunoglobulin-like Cell Adhesion Molecules01:31

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
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Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Related Experiment Video

Updated: Feb 15, 2026

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
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Nephronectin Regulates Mesangial Cell Adhesion and Behavior in Glomeruli.

Susan E Zimmerman1, Chitkale Hiremath1, Jun Tsunezumi1

  • 1Division of Nephrology, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas.

Journal of the American Society of Nephrology : JASN
|January 17, 2018
PubMed
Summary

Nephronectin (Npnt) in the glomerular basement membrane (GBM) is crucial for kidney health. Its absence increases mesangial cells, causing kidney disease, revealing a new adhesion complex vital for GBM-mesangial cell interactions.

Keywords:
cell biology and structurecell-matrix-interactionsglomerular basement membranemesangial cellsnephronectin

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Area of Science:

  • Nephrology
  • Cell Biology
  • Integrative Biology

Background:

  • The glomerulus filters blood, with the glomerular basement membrane (GBM) essential for this function.
  • Mesangial cells stabilize glomeruli, but their interaction with the GBM is poorly understood.
  • Nephronectin (Npnt), a GBM protein and α8β1 integrin ligand, is implicated in these interactions.

Purpose of the Study:

  • To investigate the role of nephronectin (Npnt) in glomerular structure and function.
  • To elucidate the molecular interactions between the GBM and mesangial cells.
  • To identify novel adhesion complexes involving Npnt and α8β1 integrin.

Main Methods:

  • Immunolocalization and in situ hybridization in adult mouse kidneys.
  • Conditional deletion of Npnt in nephron progenitors and podocytes.
  • Analysis of mesangial cell number and mesangial sclerosis.

Main Results:

  • Nephronectin is produced by podocytes and deposited in the GBM.
  • Conditional Npnt deletion increased mesangial cell number and caused mesangial sclerosis.
  • Npnt and α8β1 integrin form novel adhesion structures at mesangial cell protrusions.
  • Absence of Npnt disrupted these adhesions and led to α8β1 integrin mislocalization.

Conclusions:

  • Nephronectin plays a critical role in regulating mesangial cell behavior via a novel α8β1 integrin-dependent adhesion complex.
  • These findings reveal a new mechanism of GBM-mesangial cell interaction.
  • Dysregulation of this interaction may contribute to kidney pathology.