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Updated: Feb 15, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
Published on: November 30, 2022
Water-Soluble Fluorescent Probe with Dual Mitochondria/Lysosome Targetability for Selective Superoxide Detection in
Xiuhong Lu1,2, Zhongjian Chen3, Xiaochun Dong1
1School of Pharmacy Fudan University , 826 Zhangheng Road, Shanghai, 201203, P. R. China.
Abstract:
A novel water-soluble fluorescein-based fluorescent probe for superoxide detection was developed. The probe is fairly stable under neutral and acidic conditions. It can be used to detect superoxide both in solution with the detection limit of 2.2 μM and in living cells. Cell imaging experiments indicated that such a probe displayed good cell penetration and O2•- could be detected with PMA-stimulated HepG2 cells in both mitochondria and lysosome. Such a probe is the first dual mitochondria- and lysosome- targetable fluorescent chemodosimeter. Additionally, O2•- in intact live zebrafish embryos was successfully visualized under PMA-stimulated conditions, and the possible detection mechanism was studied as well.
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