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Let-7 miRNA Precursors Co-express with LIN28B in Cervical Cells

Aida Margarita Zamora-Contreras1,2, Luis Marat Alvarez-Salas1

  • 1Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del I.P.N., Av. I.P.N. 2508, Ciudad de Mexico 07360, Mexico.

Microrna (Shariqah, United Arab Emirates)
|January 17, 2018
PubMed
Abstract

Insights

Dysregulated let-7 microRNAs (miRNAs) are common in cervical cancer. Structural variations in pre-miRNA sequences may allow some let-7 miRNAs to evade LIN28B regulation, impacting cancer development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • let-7 microRNAs (miRNAs) are frequently dysregulated in cervical cancer.
  • LIN28 proteins regulate let-7 biogenesis by binding to pre-miRNA structures.
  • Some let-7 miRNAs may escape LIN28 regulation, a phenomenon not fully understood in cervical carcinogenesis.

Purpose of the Study:

  • To correlate pre-let-7 miRNA and LIN28B levels in cervical cell lines.
  • To investigate the impact of malignancy and HPV status on these correlations.
  • To explore potential structural mechanisms of let-7 miRNA regulation by LIN28B.

Main Methods:

  • Quantitative reverse transcription PCR (RTqPCR) for pre-let-7 levels.
  • Immunoblotting for LIN28B and let-7 targets.
  • In silico analysis of pre-let-7 sequences, structures, and expression correlations.

Main Results:

  • LIN28B was detected in all cell lines, with higher expression in tumor lines.
  • High levels of pre-let-7c/f-1 and pre-miR-98 were common, irrespective of malignancy or LIN28B levels.
  • LIN28B positively correlated with pre-let-7i/g/f-1 and pre-miR-98 in tumor lines, suggesting regulatory escape.
  • Distinct preE-stem structures in pre-let-7 miRNAs may influence LIN28B interaction and regulation.

Conclusions:

  • Differential pre-let-7 levels in cervical cell lines may stem from alternative preE structuring.
  • Altered preE structure affects LIN28B interaction, leading to differential let-7 regulation.
  • This structural variation offers a potential mechanism for let-7 dysregulation in cervical cancer progression.
Keywords:
Cervical cancerLin28Blet-7 biogenesislet-7 regulationmiRNAspre-let-7

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