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Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
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High temporal resolution delayed analysis of clinical microdialysate streams.
S A N Gowers1, K Hamaoui, P Cunnea
1Department of Bioengineering, Imperial College, London, SW7 2AZ, UK. m.boutelle@imperial.ac.uk.
The Analyst
|January 17, 2018
Summary
This study introduces a tubing method for storing clinical microdialysis samples, enabling delayed analysis up to 72 days. This technique offers an alternative to traditional sampling, preserving sample integrity for glucose and lactate measurements.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Neuroscience
Background:
- Clinical microdialysis is crucial for in vivo monitoring.
- Traditional sampling methods can limit temporal resolution.
- Delayed analysis of microdialysis samples is often required.
Purpose of the Study:
- To present a novel tubing method for storing clinical microdialysis samples.
- To enable delayed analysis of samples with high temporal resolution.
- To offer an alternative to discrete offline microdialysis sampling.
Main Methods:
- Utilizing tubing for microdialysis sample storage.
- Investigating sample stability at -80 °C for up to 72 days.
- Developing a general model for optimizing tubing parameters (storage time, flow rate, back pressure).
Main Results:
- Microdialysis samples stored in tubing remained stable for up to 72 days at -80 °C.
- The method demonstrated applicability to glucose and lactate measurements in vivo.
- A model was presented to guide tubing parameter selection, preventing probe leakage and maximizing temporal resolution.
Conclusions:
- Tubing offers a viable method for storing clinical microdialysis samples for delayed analysis.
- This approach enhances temporal resolution and sample stability in in vivo monitoring.
- The developed model aids in optimizing the microdialysis sampling process.
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