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Updated: Feb 15, 2026

Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
Published on: March 28, 2025
A graph-based approach to analyze flux-balanced pathways in metabolic networks
Mona Arabzadeh1, Morteza Saheb Zamani1, Mehdi Sedighi1
1Department of Computer Engineering and Information Technology, Amirkabir University of Technology, Tehran, Iran.
Abstract:
An elementary flux mode (EFM) is a pathway with minimum set of reactions that are functional in steady-state constrained space. Due to the high computational complexity of calculating EFMs, different approaches have been proposed to find these flux-balanced pathways. In this paper, an approach to find a subset of EFMs is proposed based on a graph data model. The given metabolic network is mapped to the graph model and decisions for reaction inclusion can be made based on metabolites and their associated reactions. This notion makes the approach more convenient to categorize the output pathways. Implications of the proposed method on metabolic networks are discussed.
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