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Updated: Feb 15, 2026

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Published on: April 17, 2011

Transfection by Electroporation.

Huntington Potter¹, Richard Heller^2,3

¹Rocky Mountain Alzheimer's Disease Center, Anschutz Medical Campus, University of Colorado, Aurora, Colorado.

Current Protocols in Molecular Biology

|January 17, 2018

Summary

This summary is machine-generated.

Electroporation uses electric shocks to insert DNA into cells, offering an easier method for gene expression and stable transformation in various cell types, including mammalian and plant cells.

Keywords:

DNA vaccine gene expression gene regulation gene therapy introduction of DNA into cells molecular biology transcription and translation

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Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Electroporation is a widely applicable method for introducing nucleic acids into cells.
It offers advantages over other transfection techniques, including ease of use and high efficiency.
This technique is crucial for genetic engineering and therapeutic applications.

Purpose of the Study:

To provide detailed protocols for electroporation of mammalian cells, including embryonic stem (ES) cells.
To describe in vivo electroporation for gene therapy and DNA vaccination.
To present modifications for plant protoplast transfection.

Main Methods:

High-voltage electric shocks are applied to cells to permeabilize membranes for DNA uptake.
Protocols cover electroporation of mammalian cells (including ES cells) and plant protoplasts.
In vivo electroporation techniques for gene therapy and DNA vaccination are detailed.

Main Results:

Electroporation demonstrates high efficiency for both stable transformation and transient gene expression.
The method is adaptable to various cell types, including mammalian and plant cells.
Successful applications include generating genetically modified mice and in vivo gene delivery.

Conclusions:

Electroporation is a versatile and efficient technique for genetic modification across diverse cell types.
Its applications range from basic research (e.g., creating knockout mice) to clinical settings (gene therapy, DNA vaccination).
The described protocols facilitate the use of electroporation in various biological research and therapeutic contexts.