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Carbon nanospheres mediated nuclear delivery of SMAR1 protein (DNA binding domain) controls breast tumor in mice

Prasad N Bhagat1, Sachin H Jadhav1, Samit Chattopadhyay2

  • 1Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India.

Nanomedicine (London, England)
|January 18, 2018
PubMed
Abstract

Insights

The DNA binding domain of SMAR1 protein (His 5), when delivered via carbon nanospheres (CNs), demonstrated significant anticancer activity. This novel approach effectively controlled breast tumor growth in a mouse model.

Area of Science:

  • Oncology
  • Nanotechnology
  • Molecular Biology

Background:

  • The SMAR1 protein plays a role in gene regulation.
  • Targeting cancer with novel drug delivery systems is crucial.

Purpose of the Study:

  • To evaluate the anticancer potential of the SMAR1 DNA binding domain (His 5).
  • To assess the efficacy of carbon nanosphere (CNs)-mediated delivery of His 5 for cancer treatment.

Main Methods:

  • His 5 was conjugated to hydrothermally synthesized carbon nanospheres (CNs).
  • In vitro and in vivo anticancer activity of CNs-His 5 was evaluated.
  • Tumor regression, 18FDG uptake, caspase assays, and IHC staining were analyzed.

Main Results:

  • CNs-His 5 significantly reduced cyclin D1 levels in MDA-MB-231 cells.
  • A 62% tumor regression was observed in tumor-bearing mice treated with CNs-His 5.
  • Tumor growth inhibition was confirmed via caspase assays and IHC staining, indicating apoptotic, antiproliferative, and antiangiogenic effects.

Conclusions:

  • The DNA binding domain of SMAR1 (His 5) exhibits potent anticancer properties.
  • CNs-mediated delivery of His 5 offers a promising strategy for controlling breast tumors.
Keywords:
DNA binding domainPETSMAR1breast cancercarbon nanospheres

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