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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Related Experiment Video

Updated: Feb 15, 2026

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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Expression and Function of Long Non-coding RNA CASC19 in Colorectal Cancer.

Jian Jun Wang1, Xiao Min Li2, Lei He2

  • 1Department of Histology and Embryology,Yijishan Hospital of Wannan Medical College,Wannan Medical College,Wuhu,Anhui 241002,China.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
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This summary is machine-generated.

Long non-coding RNA CASC19 is highly expressed in colorectal cancer (CRC) and promotes tumor metastasis. Knocking down CASC19 inhibits CRC cell migration, indicating its significance in CRC progression.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) remains a significant global health challenge.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific role of lncRNA CASC19 in CRC pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the expression levels of lncRNA CASC19 in colorectal cancer (CRC) tissues and cell lines.
  • To explore the functional significance of CASC19 in CRC cell migration and metastasis.
  • To determine the correlation between CASC19 expression and clinicopathological features of CRC.

Main Methods:

  • Real-time quantitative polymerase chain reaction (RT-qPCR) for CASC19 expression analysis.
  • Analysis of CASC19 expression in 40 paired CRC surgical specimens and 5 CRC cell lines.
  • Transwell assays to assess CRC cell migration after CASC19 knockdown using small interfering RNA (siRNA).

Main Results:

  • CASC19 expression was significantly upregulated in CRC tissues compared to adjacent normal tissues (P<0.0001).
  • Elevated CASC19 expression correlated with tumor metastasis (P=0.044), including lymph node and distant metastasis.
  • Knockdown of CASC19 significantly inhibited the in vitro migration ability of CRC cells.

Conclusions:

  • lncRNA CASC19 is overexpressed in colorectal cancer.
  • CASC19 expression is significantly associated with tumor size and metastatic potential in CRC.
  • High CASC19 expression may serve as a potential biomarker and therapeutic target for promoting CRC metastasis.