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Health status alterations in Ruditapes philippinarum after continuous secondary effluent exposure before and after
B Díaz-Garduño1, J A Perales2, C Garrido-Pérez2
1Physical Chemical Department, University Institute of Marine Research (INMAR), International Campus of Excellence of the Sea (CEI•MAR), Faculty of Marine and Environmental Sciences, University of Cadiz, 11510, Puerto Real, Cadiz, Spain.
Wastewater effluent harms marine clams, causing stress and altering energy. A microalgae photobiotreatment significantly reduced these toxic effects, protecting clam health.
Area of Science:
- Environmental Toxicology
- Marine Biology
- Wastewater Treatment
Background:
- Marine environments face risks from whole effluent toxicity.
- Wastewater treatment plants (WWTPs) discharge effluents that can act as contaminants.
- Understanding the biological impact on marine organisms is crucial.
Purpose of the Study:
- To assess the adverse effects of WWTP effluent on the marine clam Ruditapes philippinarum.
- To evaluate the efficacy of a microalgae photobiotreatment (PhtBio) in reducing effluent toxicity.
- To investigate specific biomarkers of stress in clams exposed to effluent.
Main Methods:
- A mobile pilot plant exposed clams to various effluent concentrations for seven days.
- Biomarkers analyzed included lysosomal membrane stability (LMS), total lipids (TLP), mitochondrial electron transport (MET), cyclooxygenase (COX), and acetylcholinesterase (AChE).
- The impact of photobiotreatment (PhtBio) on effluent toxicity was assessed.
Main Results:
- Effluent exposure caused significant alterations in clam health, including neuroendocrine effects, energy budget disruptions, and lipid storage changes.
- Lysosomal membrane stability, inflammatory mechanisms, and neurotoxic effects were notably impacted.
- Photobiotreatment demonstrated a reduction in the effluent's toxic effects on the exposed organisms.
Conclusions:
- Wastewater effluent poses a significant stress risk to marine clam health.
- Biomarkers like LMS, TLP, MET, COX, and AChE are effective indicators of effluent toxicity.
- Photobiotreatment shows promise as an effective tertiary treatment for mitigating effluent-induced harm to marine life.
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