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MicroRNA-95 promotes myogenic differentiation by down-regulation of aminoacyl-tRNA synthase complex-interacting

Biao Li1, Shanshan Xie1, Chunbo Cai1

  • 1Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.

Oncotarget
|January 18, 2018
PubMed

Insights

MicroRNA-95 (miR-95) promotes skeletal muscle development by enhancing myogenic differentiation. It achieves this by down-regulating aminoacyl-tRNA synthase complex-interacting multifunctional protein 2 (AIMP2) expression in C2C12 myoblasts.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • MicroRNA-95 (miR-95) is known to promote cancer cell proliferation.
  • Its role in skeletal muscle development was previously unreported.
  • Genetically engineered pigs with a myostatin (MSTN) loss-of-function mutation (MSTN-/-) exhibit a double muscle phenotype.

Purpose of the Study:

  • To investigate the function of miR-95 in myogenic differentiation.
  • To explore the potential role of miR-95 in skeletal muscle development.

Main Methods:

  • Utilized a murine myoblast cell line (C2C12).
  • Performed bioinformatical analysis and luciferase reporter gene assays.
  • Employed small interfering RNA (siRNA) to inhibit endogenous AIMP2 expression.

Main Results:

  • miR-95 expression was upregulated in MSTN-/- pigs.
  • miR-95 regulates the expression of myogenic differentiation markers (myosin heavy chain and myogenin).
  • AIMP2 was identified as a direct target of miR-95, with higher miR-95 leading to lower AIMP2 protein levels.
  • AIMP2 inhibition by siRNA increased the expression of myogenic differentiation markers.

Conclusions:

  • miR-95 promotes myogenic differentiation in C2C12 myoblasts.
  • miR-95 likely plays a positive role in skeletal muscle development.
  • This occurs through the downregulation of AIMP2 protein expression.
Keywords:
AIMP2C2C12differentiationmiR-95myostatin

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