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Updated: Feb 15, 2026

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
Published on: May 19, 2014
Time collection and storage conditions of lipid profile
C N França1,2, C C Mendes3, C E S Ferreira3,4
1Pós Graduação em Ciências da Saúde, Universidade Santo Amaro, São Paulo, SP, Brasil.
Abstract:
The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.
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