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Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
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Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures

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Dynamic Susceptibility Contrast MRI in Small Animals

Pilar López-Larrubia1

  • 1Instituto de Investigaciones Biomédicas "Alberto Sols", CSIC-UAM, Madrid, Spain. plopez@iib.uam.es.

Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2018
PubMed

Insights

Magnetic resonance imaging (MRI) is a powerful tool for studying brain blood flow. This chapter details dynamic susceptibility contrast MRI methods for preclinical research in small animals to understand brain diseases.

Area of Science:

  • Neuroimaging
  • Biomedical Engineering
  • Radiology

Background:

  • Magnetic resonance imaging (MRI) is established for studying cerebral perfusion in clinical and research settings.
  • Perfusion MRI is valuable for assessing brain diseases like tumors, neurodegeneration, and injuries.
  • In preclinical research, perfusion MRI aids in characterizing disease models and validating therapies.

Purpose of the Study:

  • To present the theoretical foundations of dynamic susceptibility contrast (DSC) MRI.
  • To outline experimental protocols for DSC MRI in small animal models.
  • To facilitate the development of perfusion MRI studies in preclinical research.

Main Methods:

  • Dynamic Susceptibility Contrast (DSC) MRI acquisition principles.
  • Theoretical basis of contrast agent dynamics in cerebral vasculature.
  • Experimental protocols tailored for small animal imaging.

Main Results:

  • Provides a comprehensive overview of DSC-MRI techniques for small animals.
  • Establishes a framework for preclinical perfusion studies.
  • Highlights the utility of perfusion MRI in disease modeling and therapeutic validation.

Conclusions:

  • Dynamic susceptibility contrast MRI is a key technique for preclinical brain research.
  • This chapter equips researchers with the knowledge to implement perfusion MRI studies in small animals.
  • Perfusion MRI is crucial for advancing our understanding and treatment of neurological disorders.
Keywords:
Animal modelBolus trackingBrain perfusionCerebral blood flowCerebral blood volumeDynamic susceptibility contrastMean transit timePreclinical MRI

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