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Assessment of Blood Brain Barrier Leakage with Gadolinium-Enhanced MRI

Min-Chi Ku1, Sonia Waiczies1, Thoralf Niendorf1,2

  • 1Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2018
PubMed

Insights

Magnetic resonance imaging (MRI) can monitor blood-brain barrier (BBB) integrity. This study presents an MRI method to detect BBB leakage in mouse models, aiding research in neurological diseases and drug effects.

Area of Science:

  • Neuroimaging
  • Biomedical Engineering
  • Pharmacology

Background:

  • The blood-brain barrier (BBB) protects the brain but its disruption is key in neurological diseases.
  • Gadolinium-based contrast agents (CAs) are used with MRI to detect BBB leakage.
  • Current methods face limitations in sensitivity and specificity for BBB permeability assessment.

Purpose of the Study:

  • To present a novel MRI method for noninvasive assessment of blood-brain barrier (BBB) integrity.
  • To demonstrate the utility of this method in preclinical disease models.
  • To evaluate its potential for assessing drug effects on BBB permeability.

Main Methods:

  • Utilizing T1-weighted MRI sequences acquired before and after contrast agent (CA) administration.
  • Applying the method in vivo in mouse models.
  • Analyzing image data to quantify CA extravasation as an indicator of BBB permeability.

Main Results:

  • The described MRI method effectively detects and quantifies BBB leakage in mouse models.
  • Demonstrated sensitivity to partial BBB compromise.
  • Showcased applicability across various experimental conditions, including neuroinflammation models.

Conclusions:

  • This T1-weighted MRI technique offers a noninvasive approach for monitoring BBB integrity.
  • It is a valuable tool for studying neurological diseases and evaluating therapeutic interventions.
  • The method's adaptability makes it suitable for diverse research applications in neuroscience and drug development.
Keywords:
Blood brain barrier (BBB)Magnetic resonance imaging (MRI)Mousecontrast agent (CA)

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