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11:34
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Published on: August 9, 2019
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Trans-acting translational regulatory RNA binding proteins.
Robert F Harvey1, Tom S Smith2, Thomas Mulroney1
1MRC Toxicology Unit, Leicester, UK.
Wiley Interdisciplinary Reviews. RNA
|January 18, 2018
Summary
This review explores novel RNA-binding proteins (RBPs) and their roles in mRNA translation regulation. Understanding these proteins and their interactions is crucial for deciphering cell fate determination.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Canonical translation machinery is well-defined.
- Noncanonical RNA-binding proteins (RBPs) regulate mRNA-specific translation.
- Emerging evidence highlights trans-acting ribosomal proteins in mRNA translation.
Purpose of the Study:
- To review methodologies for identifying novel RBPs and their functions in translation.
- To discuss protein domains and RNA motifs involved in RNA-binding.
- To elucidate the role of trans-acting ribosomal proteins in specific mRNA translation.
Main Methods:
- Review of recent methodologies for RBP identification.
- Analysis of protein domains and RNA motifs.
- Discussion of RBP combinatorial codes in cellular regulation.
Main Results:
- Numerous novel RBPs have been identified, but their functions remain largely unknown.
- RBPs form combinatorial complexes to dictate cell fate through mRNA translation.
- Trans-acting ribosomal proteins play a key role in directing specific mRNA translation.
Conclusions:
- Further research is needed to address shortcomings in current methodologies for studying translation.
- A deeper understanding of RBPs and their interactions is essential for advancing translational control research.
- Elucidating the function of novel RBPs will enhance our comprehension of cell fate determination.
Keywords:
IRES uORFRNA binding proteininteractome capturemRNA translationprotein synthesisterminal oligopyrimidine tractMore Related Videos
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