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2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition
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Microbiome associated with denture malodour.

S Yitzhaki1, L Reshef2, U Gophna2

  • 1Department of Prosthodontics, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv 6997801, Israel.

Journal of Breath Research
|January 18, 2018
PubMed
Summary

Next-generation sequencing reveals distinct oral microbiomes in denture malodour. Malodourous dentures harbor higher bacterial diversity and specific microbial profiles, offering insights into this common issue.

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Area of Science:

  • Oral microbiology
  • Geriatric dentistry
  • Microbiome analysis

Background:

  • Culturing limitations historically hindered understanding of oral microbial ecosystems.
  • Increasing life expectancy drives focus on denture malodour in geriatric populations.

Purpose of the Study:

  • To investigate the oral microbiome associated with denture malodour using advanced sequencing techniques.
  • To compare microbial composition and diversity between malodourous and non-malodourous dentures.

Main Methods:

  • Utilized next-generation deep sequencing (16S rDNA technology) on DNA from denture swab samples.
  • Analyzed samples from 26 full denture patients, classifying taxa into phyla, genera, and species.
  • Organoleptically assessed denture malodour, categorizing samples as malodour positive (score ≥ 2).

Main Results:

  • Identified nine phyla, 29 genera, and 117 species.
  • Malodour positive samples showed increased abundance of Firmicutes, Fusobacteria, and specific genera (Leptotrichia, Atopobium, Megasphaera, Oribacterium, Campylobacter).
  • Demonstrated higher bacterial diversity and significant differences in microbial profiles in malodourous samples.

Conclusions:

  • The oral microbial populations of malodourous and non-malodourous dentures differ significantly in composition and diversity.
  • Deep sequencing provides a comprehensive view of the denture microbiome, crucial for understanding malodour.
  • Findings contribute to the growing knowledge of oral ecosystems in aging populations.