Related Experiment Video
Updated: Jul 20, 2026
06:02
2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition
Published on: December 26, 2016
10.9K
Microbiome associated with denture malodour.
S Yitzhaki1, L Reshef2, U Gophna2
1Department of Prosthodontics, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv 6997801, Israel.
Journal of Breath Research
|January 18, 2018
Summary
Next-generation sequencing reveals distinct oral microbiomes in denture malodour. Malodourous dentures harbor higher bacterial diversity and specific microbial profiles, offering insights into this common issue.
Area of Science:
- Oral microbiology
- Geriatric dentistry
- Microbiome analysis
Background:
- Culturing limitations historically hindered understanding of oral microbial ecosystems.
- Increasing life expectancy drives focus on denture malodour in geriatric populations.
Purpose of the Study:
- To investigate the oral microbiome associated with denture malodour using advanced sequencing techniques.
- To compare microbial composition and diversity between malodourous and non-malodourous dentures.
Main Methods:
- Utilized next-generation deep sequencing (16S rDNA technology) on DNA from denture swab samples.
- Analyzed samples from 26 full denture patients, classifying taxa into phyla, genera, and species.
- Organoleptically assessed denture malodour, categorizing samples as malodour positive (score ≥ 2).
Main Results:
- Identified nine phyla, 29 genera, and 117 species.
- Malodour positive samples showed increased abundance of Firmicutes, Fusobacteria, and specific genera (Leptotrichia, Atopobium, Megasphaera, Oribacterium, Campylobacter).
- Demonstrated higher bacterial diversity and significant differences in microbial profiles in malodourous samples.
Conclusions:
- The oral microbial populations of malodourous and non-malodourous dentures differ significantly in composition and diversity.
- Deep sequencing provides a comprehensive view of the denture microbiome, crucial for understanding malodour.
- Findings contribute to the growing knowledge of oral ecosystems in aging populations.
Related Concept Videos
01:27The Skin Microbiota
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
01:22Microbiome of the Eye
The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
01:27The Oral Microbiota
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
01:28Development of the Oral Microbiota
The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...
01:29Microbiota of the Respiratory Tract
The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more like...
01:28Microbiota of the Urogenital Tract
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...