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Published on: August 25, 2018
Copy Number Variation in SOX6 Contributes to Chicken Muscle Development
Shudai Lin1, Xiran Lin2, Zihao Zhang3
1Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science of South China Agricultural University, Guangzhou 510642, China. shudailin@stu.scau.edu.cn.
Copy number variations (CNVs) in chickens are linked to the SOX6 gene, impacting skeletal muscle development. Increased SOX6 expression, driven by CNVs, promotes muscle cell growth and differentiation.
Area of Science:
- Genetics
- Animal Science
- Molecular Biology
Background:
- Copy number variations (CNVs) are associated with complex traits and diseases.
- The SOX6 gene is crucial for muscle fiber differentiation in other species, but its role in chickens is unclear.
Purpose of the Study:
- To investigate the role of SOX6 in chicken skeletal muscle development.
- To identify genetic variations, specifically CNVs, associated with muscle traits in chickens.
Main Methods:
- AccuCopy® and CNVplex® analyses were used to identify CNVs.
- Quantitative real-time polymerase chain reaction (qRT-PCR) assessed SOX6 mRNA expression.
- SOX6 gene knockdown was performed to evaluate its downstream effects.
Main Results:
- Two CNVs, including CNP13 overlapping SOX6, were significantly associated with chicken traits.
- SOX6 mRNA expression was positively correlated with CNV count and high during muscle differentiation.
- Knockdown of SOX6 led to down-regulation of key muscle growth-related genes (IGFIR1, MYF6, SOX9, SHOX, CCND1).
Conclusions:
- CNV count at CNP13 positively influences SOX6 expression in chickens.
- SOX6 promotes skeletal muscle cell proliferation and differentiation by up-regulating muscle growth genes.
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