Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acidic glycoproteins from bovine compact bone.

S Sato, F Rahemtulla, C W Prince

    Connective Tissue Research
    |January 1, 1985
    PubMed
    Summary

    Researchers isolated six acidic glycoproteins from bovine bone, including bone sialoprotein (BSP) and osteonectin. Antibodies to osteonectin also recognized 80K and 30K glycoproteins, revealing shared characteristics in bone matrix proteins.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Treatment of IgA nephropathy].

    Vnitrni lekarstvi·2008
    Same author

    Role of aberrant glycosylation of IgA1 molecules in the pathogenesis of IgA nephropathy.

    Kidney & blood pressure research·2008
    Same author

    Patients with IgA nephropathy have increased serum galactose-deficient IgA1 levels.

    Kidney international·2007
    Same author

    [Immunoglobulin A and renal diseases].

    Vnitrni lekarstvi·2006
    Same author

    Heterogeneity of IgG glycosylation in adult periodontal disease.

    Journal of dental research·2005
    Same author

    [IgA nephropathy. Significance of immunoglobulin A glycosylation in pathogenesis and clinical presentation].

    Casopis lekaru ceskych·2003

    Area of Science:

    • Biochemistry
    • Biomaterials Science
    • Molecular Biology

    Background:

    • Bone is a complex matrix containing various non-collagenous proteins.
    • Acidic glycoproteins play crucial roles in bone mineralization and cell interactions.

    Purpose of the Study:

    • To isolate and characterize acidic glycoproteins from adult bovine compact bone.
    • To investigate the immunological relationships between these bone glycoproteins.

    Main Methods:

    • Isolation of glycoproteins using techniques like affinity chromatography (Blue Sepharose CL-6B).
    • Analysis of amino acid and carbohydrate composition.
    • Production of antibodies against purified osteonectin.
    • Enzyme-linked immunosorbent assays (ELISA) for cross-reactivity studies.

    Main Results:

    • Six acidic glycoproteins (85K-BSP, 80K, 67K, 60K, 40K-osteonectin, 30K) were isolated.
    • Proteins showed high aspartic and glutamic acid content; varying carbohydrate compositions were observed.
    • Antibodies to osteonectin cross-reacted with 80K and 30K glycoproteins, indicating structural similarities.

    Conclusions:

    • Bovine compact bone contains a family of acidic glycoproteins with distinct molecular weights and compositions.
    • Osteonectin shares antigenic epitopes with 80K and 30K bone glycoproteins.
    • These findings contribute to understanding the molecular composition and organization of the bone extracellular matrix.

    Related Experiment Videos