Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

25.3K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

5.2K
5.2K
RNA Splicing01:32

RNA Splicing

60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
Null and Alternative Hypotheses01:16

Null and Alternative Hypotheses

12.9K
The actual hypothesis testing begins by considering two hypotheses. They are termed  the null hypothesis and the alternative hypothesis. These hypotheses contain opposing viewpoints.
The null hypothesis, denoted by H0 is a statement of no difference between the variables—they are not related. This can often be considered the status quo. As  a result if you cannot accept the null, it requires some action.
The alternative hypothesis, denoted by H1 or Ha, is a claim about the...
12.9K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.5K
3.5K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

7.0K
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective growth of chirality-enriched semiconducting carbon nanotubes by using bimetallic catalysts from salt precursors.

Nanoscale·2018
Same author

MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions.

Molecular plant·2018
Same author

A novel uromodulin mutation in autosomal dominant tubulointerstitial kidney disease: a pedigree-based study and literature review.

Renal failure·2018
Same author

Dietary myo-inositol deficiency decreased intestinal immune function related to NF-κB and TOR signaling in the intestine of young grass carp (Ctenopharyngodon idella).

Fish & shellfish immunology·2018
Same author

A Review of Ginseng Clinical Trials Registered in the WHO International Clinical Trials Registry Platform.

BioMed research international·2018
Same author

A Facile Approach to Prepare Multiple Heteroatom-Doped Carbon Materials from Imine-Linked Porous Organic Polymers.

Scientific reports·2018
Same journal

Inflammatory Preconditioning Reprograms Mesenchymal Stromal Cell-Derived Extracellular Vesicles into Distinct Functional States for Immunomodulation and Wound Healing.

Stem cells and development·2026
Same journal

Cancer Stem Cells as Dynamic Attractor States: Systems Biology of Tumor Plasticity and Therapy Resistance.

Stem cells and development·2026
Same journal

Evaluation of Puromycin-Induced Podocyte Injury and Protective Effects of Voclosporin Using Induced Pluripotent Stem Cells from a Patient with Nephrotic Syndrome Harboring an <i>INF2</i> Variant.

Stem cells and development·2026
Same journal

Simplified Derivation of Stable Early Passage Murine Embryonic and Parthenogenetic Stem Cells.

Stem cells and development·2026
Same journal

OCT3/4-Enhanced Dental Mesenchymal Stem Cells Acquire Hematopoietic Stem Cell-Like Properties and Support Hematopoietic Recovery in a Myelosuppressed Mouse Model.

Stem cells and development·2026
Same journal

Hematopoietic Stem Cell-Based Cell and Gene Therapy Beyond Monogenic Diseases.

Stem cells and development·2026
See all related articles

Related Experiment Video

Updated: Feb 15, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.4K

PAX6 Alternative Splicing and Corneal Development.

Jung Woo Park1, Juan Yang1, Ren-He Xu1

  • 1Faculty of Health Sciences, University of Macau , Macau, China .

Stem Cells and Development
|January 19, 2018
PubMed
Summary
This summary is machine-generated.

Paired box protein 6 (PAX6) is crucial for eye development. Alternative splicing of PAX6 may significantly impact corneal development, a role previously overlooked.

Keywords:
Pax6 splicing regulationalternative splicingcorneal development

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.5K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.5K

Related Experiment Videos

Last Updated: Feb 15, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.4K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.5K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.5K

Area of Science:

  • Developmental biology
  • Molecular genetics
  • Ophthalmology

Background:

  • Paired box protein 6 (PAX6) is a key regulator of eye development.
  • Research has primarily focused on the transcriptional control of PAX6.
  • Alternative splicing (AS) as a regulatory mechanism in eye development remains understudied.

Purpose of the Study:

  • To investigate the role of alternative splicing (AS) in Pax6 gene regulation during eye development.
  • To explore the potential significance of different PAX6 isoforms in corneal development.

Main Methods:

  • Analysis of Pax6 pre-mRNA splicing patterns.
  • Identification and characterization of PAX6 isoforms.
  • Functional studies in ocular development models (details not provided in abstract).

Main Results:

  • Two distinct PAX6 isoforms are generated through AS of Pax6 pre-mRNA.
  • These PAX6 isoforms may play critical, previously unrecognized roles in eye development.
  • Evidence suggests a particular importance in corneal development.

Conclusions:

  • Alternative splicing of PAX6 is an important regulatory mechanism in eye development.
  • PAX6 isoforms generated by AS have a significant, underappreciated role, especially in corneal development.
  • Further research into PAX6 AS is warranted to fully understand eye development.