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miR-218 inhibits acute promyelocytic leukemia cell growth by targeting BMI-1

Yan Wang1, Hai-Hong Sun2, Ming-Hua Sui3

  • 1Department of Hematology, Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.

Oncology Letters
|January 19, 2018
PubMed

Insights

MicroRNA-218 (miR-218) acts as a tumor suppressor in acute promyelocytic leukemia (APL). Its downregulation in APL suggests the miR-218/BMI-1 axis as a potential therapeutic target.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Acute promyelocytic leukemia (APL) is a distinct subtype of acute myeloid leukemia.
  • MicroRNAs (miRs) have demonstrated therapeutic potential in APL.
  • The specific role of miR-218 in APL pathogenesis was previously undefined.

Purpose of the Study:

  • To investigate the expression levels of miR-218 in APL.
  • To determine the functional impact of miR-218 on HL-60 cell viability and proliferation.
  • To elucidate the relationship between miR-218 and BMI-1 in APL.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to assess miR-218 expression.
  • HL-60 cells were utilized to study the effects of miR-218 overexpression.
  • Western blotting and RT-qPCR were employed to analyze BMI-1 expression.

Main Results:

  • miR-218 was found to be significantly downregulated in APL marrow tissues compared to normal tissues.
  • Overexpression of miR-218 suppressed HL-60 cell proliferation, induced G0/G1 cell cycle arrest, and promoted apoptosis.
  • miR-218 expression negatively correlated with BMI-1 (B-cell-specific Moloney murine leukemia virus integration site 1) mRNA and protein levels, which were reduced upon miR-218 mimic transfection.

Conclusions:

  • miR-218 functions as a tumor suppressor in APL.
  • The miR-218/BMI-1 signaling pathway represents a potential diagnostic biomarker and therapeutic target for APL treatment.
Keywords:
B-cell-specific Moloney murine leukemia virus integration site 1acute promyelocytic leukemiaapoptosiscell cyclesmicroRNA-218proliferation

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