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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.0K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

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The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
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Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Updated: Feb 15, 2026

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
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Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

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Break Breast Cancer Addiction by CRISPR/Cas9 Genome Editing.

Haitao Yang1, MariaLynn Jaeger2, Averi Walker2

  • 1Laboratory for Cancer Genome Editing, Zhuhai Lifecode Medical Technologies. Inc. Department of Prenatal Diagnosis, Huizhou 2nd Hospital for Children and Women, #101 University Road, Tangjiawan, Zhuhai, 518900, Guangdong, China.

Journal of Cancer
|January 19, 2018
PubMed
Summary

CRISPR/Cas genome editing advances biomarker discovery for breast cancer personalized medicine. This review explores its impact on identifying cancer programs driving tumorigenesis, metastasis, and treatment resistance.

Keywords:
Breast cancerCRISPR/Cas9anticancer resistancegenome.metastasismutationsprotein degradationtranscriptiontumorigenesis

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Area of Science:

  • Oncology
  • Genomics
  • Biotechnology

Background:

  • Breast cancer is a leading global cancer in women.
  • Aberrant gene expression and protein degradation drive cancer evolution, metastasis, and treatment resistance.
  • Cancer cells develop dependencies on specific cellular programs for uncontrolled growth.

Purpose of the Study:

  • To review the role of CRISPR/Cas genome editing in identifying and validating anticancer biomarkers for breast cancer.
  • To explore the application of CRISPR/Cas9 in understanding breast cancer genome.
  • To highlight the potential of these biomarkers in personalized medicine.

Main Methods:

  • Review of CRISPR/Cas9-mediated genome editing applications in breast cancer research.
  • Analysis of studies focusing on somatic genome editing, transcription, and protein degradation.
  • Examination of CRISPR/Cas9's role in identifying cancer-specific dependencies.

Main Results:

  • CRISPR/Cas9 technology is instrumental in dissecting cancer-driving gene expression and protein degradation pathways.
  • Genome editing facilitates the identification of cellular programs crucial for breast cancer progression.
  • Validation of potential biomarkers for personalized breast cancer treatment strategies.

Conclusions:

  • CRISPR/Cas-mediated genome editing is a powerful tool for discovering breast cancer biomarkers.
  • Understanding cancer-specific dependencies through genome editing can lead to novel therapeutic targets.
  • This approach holds significant promise for advancing personalized medicine in breast cancer treatment.