Influence of highly concentrated fluoride dentifrices on remineralization characteristics of enamel in vitro

The aim of this in vitro study was to evaluate the role of highly fluoridated dentifrice on remineralization characteristics of lowly and highly pre-demineralized enamel artificial caries lesions.

Bovine enamel specimens were prepared (pH 4.95; 21 days) and discriminated in either lowly [L] or highly [H] pre-demineralized artificial caries lesions. Specimens with a mean ΔZ_baseline,L (95% CI) of 5120 (4995; 5245) vol.% × μm and a mean ΔZ_baseline,H of 8187 (8036; 8339) vol.% × μm were selected and randomly allocated to 12 groups (n = 20). Treatments during pH-cycling (28 days; 6 × 60 min demineralization/day) were brushing 2×/day with fluoride-free (0 ppm F^- [L₀/H₀]), 1100 ppm F^- [L₁₁₀₀/H₁₁₀₀], 2800 ppm F^- [L₂₈₀₀/H₂₈₀₀], 5000 ppm F^- [L₅₀₀₀/H₅₀₀₀], 5000 ppm F^- + glycerin [L_{5000 + glycerin}/H_{5000 + glycerin}], and 5000 ppm F^- + TCP [L_{5000 + TCP}/H_{5000 + TCP}] containing dentifrices. Dentifrice slurries were prepared with deionized water (1:3wt/wt). After cycling specimens presenting lesion surface loss were discarded and for the remaining 202 specimens, transversal microradiographic (TMR) analyses (ΔZ_pH-cycle/LD_pH-cycle) were performed again. Changes in mineral loss (ΔΔZ = ΔZ_baseline - ΔZ_pH-cycle) and lesion depth (ΔLD = LD_baseline - LD_pH-cycle) were calculated.

Significant differences for ΔΔZ could be found between L₀, L₁₁₀₀, and L₅₀₀₀ as well as H₀, H₁₁₀₀, and H₂₈₀₀/H₅₀₀₀ (p ≤ 0.01; ANCOVA). Except for 0 ppm F^-, higher ΔΔZ could be found in highly compared with lowly demineralized specimens (p ≤ 0.004; ANCOVA). After pH-cycling, a second lesion front could only be observed in H₅₀₀₀ and H_{5000 + TCP}. The correlation between ΔΔZ and F^- was moderate for lowly and highly demineralized lesions (r_L = 0.591; p_L < 0.001; r_H = 0.746; p_H < 0.001), indicating a fluoride dose response for both.

For both baseline substrate conditions, a dose response for fluoride could be revealed.

Remineralization characteristics of enamel directly depended on baseline mineral loss.