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Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores

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Interaction network of core ABA signaling components in maize.

Ying-Ge Wang¹, Feng-Ling Fu¹, Hao-Qiang Yu¹

¹Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.

Plant Molecular Biology

|January 19, 2018

Summary

This study defines the core abscisic acid (ABA) signaling network in maize, identifying key ABA receptors, phosphatases, and kinases. It reveals novel ABA signaling components and substrates, enhancing our understanding of plant stress responses.

Keywords:

ABA signaling Maize Signaling network ZmPP2Cs ZmPYLs ZmSnRK2s

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Area of Science:

Plant Biology
Molecular Biology
Biochemistry

Background:

Abscisic acid (ABA) is crucial for plant development and stress responses.
The ABA signaling pathway involves PYL receptors, PP2C phosphatases, and SnRK2 kinases.
This pathway is well-characterized in Arabidopsis but less understood in monocot maize.

Purpose of the Study:

To define the comprehensive core ABA signaling network in monocot maize.
To investigate the gene expression, localization, and interactions of key ABA signaling components in maize.
To identify novel ABA signaling substrates in maize.

Main Methods:

Utilized Arabidopsis mutants (pyl112458, snrk2.2/3/6) to identify maize ABA signaling components.
Analyzed gene expression, subcellular localization, and protein-protein interactions of ZmPYLs, ZmPP2Cs, and ZmSnRK2s.
Employed mass spectrometry to isolate putative ZmSnRK2 substrates.

Main Results:

Identified ABA-responsive ZmPYLs, ZmPP2Cs, and ZmSnRK2s in maize.
Characterized the interaction network and ABA dependency of PYL-PP2C interactions.
Discovered 69 putative ZmSnRK2 substrates, including 23 novel phosphoproteins not found in Arabidopsis.

Conclusions:

A comprehensive core ABA signaling network in maize has been defined.
This research provides new insights into ABA signaling mechanisms in monocots.
The findings contribute to understanding plant adaptation to abiotic stress.