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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
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Related Experiment Video

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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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A simple protocol for transfecting human mesenchymal stem cells.

Talita Giacomet de Carvalho1,2, Felipe Matheus Pellenz1, Alvaro Laureano3

  • 1Gene Therapy Center, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, 90035-903, Brazil.

Biotechnology Letters
|January 19, 2018
PubMed
Summary
This summary is machine-generated.

Developing a simple, safe, and rapid protocol for mesenchymal stromal cells (MSCs) transfection is crucial for cell and gene therapy. This study optimized non-viral transfection for efficient gene delivery in MSCs.

Keywords:
Gene therapyMesenchymal stem cellTransfection

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Area of Science:

  • Cell Biology
  • Regenerative Medicine
  • Gene Therapy

Background:

  • Mesenchymal stromal cells (MSCs) are multipotent stem cells with potential in cell and gene therapy.
  • Bone marrow-derived MSCs are ideal for gene delivery due to differentiation and expansion capabilities.
  • Efficient transfection of MSCs is challenging, hindering therapeutic applications.

Purpose of the Study:

  • To develop a standardized, simple, and efficient protocol for mesenchymal stromal cells (MSCs) transfection.
  • To achieve high transfection efficiency without viral vectors or specialized equipment.
  • To optimize non-viral gene delivery methods for MSCs.

Main Methods:

  • Systematic experimentation to establish a transfection protocol for MSCs.
  • Utilized Lipofectamine 3000® with a specific lipid/DNA ratio.
  • Focused on early passage human MSCs for transfection optimization.

Main Results:

  • A rapid, simple, and safe protocol for MSCs transfection was established.
  • Achieved significant transfection efficiencies of up to 26% in human MSCs.
  • The protocol avoids viral particles and expensive, specialized equipment.

Conclusions:

  • Lipofectamine 3000® mediated transfection at a 3.0 µL/µg lipid/DNA ratio is effective for human MSCs.
  • The developed protocol offers a practical approach for MSC gene therapy.
  • Optimized non-viral transfection enhances the therapeutic potential of MSCs.