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Related Concept Videos

Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids02:43

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Nucleic Acids02:43

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Nucleic Acid Structure01:25

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Nucleic Acids and Nucleotides01:20

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
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Biosynthesis of Nucleic Acids01:28

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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
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Related Experiment Video

Updated: Feb 15, 2026

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
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Laser Microdissection Workflow for Isolating Nucleic Acids from Fixed and Frozen Tissue Samples.

Yelena G Golubeva1, Andrew C Warner2

  • 1Cancer Genomic Research Laboratory (CGR), Division of Cancer Epidemiology and Genetics, NCI, FNLCR, Leidos Biomedical Research, Inc, Gaithersburg, MD, USA. yelena.golubeva@nih.gov.

Methods in Molecular Biology (Clifton, N.J.)
|January 19, 2018
PubMed
Summary
This summary is machine-generated.

Laser Capture Microdissection (LCM) isolates specific cells for precise molecular analysis. This technique enhances biomarker discovery and personalized medicine by enriching genomic samples from diverse tissues.

Keywords:
DNAFFPE tissueFrozen tissueIR laserLCM workflowLarge-scale LCM projectLaser capture microdissectionMolecular analysisMolecular fixativesRNAUV laser

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Area of Science:

  • Histology and Molecular Biology
  • Medical Research
  • Genomic Sample Enrichment

Background:

  • Laser Capture Microdissection (LCM) is a key technique bridging histology and molecular biology.
  • LCM enables the isolation of specific cell populations from their surrounding environment.
  • Genomic sample enrichment via LCM enhances sensitivity and precision in molecular assays.

Purpose of the Study:

  • To provide insights into the design and execution of diverse Laser Capture Microdissection projects.
  • To outline considerations for planning microdissection studies.
  • To present standardized workflows and protocols for various tissues and downstream analyses.

Main Methods:

  • Standardized protocols for Laser Capture Microdissection (LCM).
  • Adaptation of workflows for diverse research targets including clinical and animal tissues, cell pellets, and archival slides.
  • Integration with various downstream molecular analysis platforms.

Main Results:

  • Successful execution of numerous diverse microdissection projects.
  • Development of standardized LCM protocols applicable to a wide range of animal and human tissues.
  • Adaptable workflows for integration with downstream analysis platforms.

Conclusions:

  • Laser Capture Microdissection is an invaluable tool for medical research, particularly in biomarker discovery and personalized medicine.
  • Standardized protocols and adaptable workflows are crucial for the successful application of LCM across diverse research objectives.
  • LCM facilitates precise molecular analysis essential for understanding disease onset, progression, and therapeutic development.