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MicroRNA-1271 functions as a metastasis and epithelial-mesenchymal transition inhibitor in human HCC by targeting the

Chao Li1, Yezhen Jiang1, Runchen Miao1

  • 1Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.

International Journal of Oncology
|January 19, 2018
PubMed

Insights

MicroRNA-1271 (miR-1271) inhibits hepatocellular carcinoma (HCC) metastasis by targeting the PTP4A1/c-Src pathway. Lower miR-1271 levels correlate with advanced HCC, indicating its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) metastasis is a major cause of mortality.
  • MicroRNAs (miRNAs) play crucial roles in regulating cancer progression, including HCC metastasis.
  • The specific role of miR-1271 in HCC metastasis requires further elucidation.

Purpose of the Study:

  • To investigate the function of miR-1271 in hepatocellular carcinoma (HCC) metastasis.
  • To identify the downstream targets and signaling pathways regulated by miR-1271 in HCC.
  • To evaluate the potential of miR-1271 as a therapeutic target for HCC.

Main Methods:

  • In vitro and in vivo assays were used to assess HCC cell migration, invasion, and metastasis.
  • Quantitative real-time PCR and Western blotting were employed to analyze gene and protein expression.
  • Luciferase reporter assays and rescue experiments were conducted to validate target genes.

Main Results:

  • Downregulation of miR-1271 was significantly associated with venous infiltration, advanced TNM stage, and shorter survival in HCC patients.
  • miR-1271 suppressed HCC cell migration, invasion, and lung metastasis formation in vitro and in vivo.
  • miR-1271 inhibited epithelial-mesenchymal transition (EMT) in HCC cells.
  • Protein tyrosine phosphatase type IVA member 1 (PTP4A1) was identified as a direct target of miR-1271.
  • miR-1271 inhibited PTP4A1-mediated phosphorylation of c-Src at Tyr416, thereby suppressing HCC metastasis.

Conclusions:

  • miR-1271 functions as a tumor suppressor in HCC metastasis.
  • miR-1271 inhibits HCC metastasis by targeting the PTP4A1/c-Src signaling pathway.
  • miR-1271 represents a promising therapeutic target for inhibiting HCC metastasis.

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