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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Overview of Electron Microscopy01:25

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Scanning Electron Microscopy01:07

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Transmission Electron Microscopy01:15

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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Immunogold Electron Microscopy01:20

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
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An Open-Source Storage Solution for Cryo-Electron Microscopy Samples.

Eveline Ultee1, Fred Schenkel2, Wen Yang1

  • 11Institute of Biology,Leiden University of Leiden,2333 BE Leiden,The Netherlands.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|January 19, 2018
PubMed
Summary
This summary is machine-generated.

A new cryo-storage system improves sample management for cryo-electron microscopy (cryo-EM) users. This efficient, organized, and cost-effective solution addresses limited access to state-of-the-art cryo-EM facilities and reduces sample wait times.

Keywords:
cryo-electron microscopygrid boxespucksstorage dewar

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Area of Science:

  • Structural Biology
  • Biophysics
  • Microscopy

Background:

  • Cryo-electron microscopy (cryo-EM) is a powerful technique for high-resolution structural analysis of biological molecules.
  • The rapid growth of cryo-EM is driven by advances in instrumentation and data processing.
  • Limited access to state-of-the-art cryo-EM facilities leads to significant wait times for researchers.

Purpose of the Study:

  • To develop an improved cryo-storage system for biological samples used in cryo-EM.
  • To enhance sample organization and accessibility within cryo-EM laboratories.
  • To provide a cost-effective and adaptable solution for managing cryo-EM samples.

Main Methods:

  • Development of a novel cryo-storage system.
  • Design focused on high storage capacity and efficient organization.
  • Adaptability for various grid storage boxes, dewars, and laboratory sizes.

Main Results:

  • The developed system offers efficient and highly organized sample storage.
  • It is simple to use and cost-effective.
  • The system is adaptable to diverse cryo-EM laboratory setups.

Conclusions:

  • The new cryo-storage system effectively addresses the challenges of sample management in cryo-EM.
  • It offers a practical solution to improve workflow efficiency and accessibility for researchers.
  • This innovation supports the growing demand for cryo-EM structural studies.