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Concrete mixing ensures a homogenous blend where aggregates are well-coated with cement paste. Concrete mixing is typically done using two main types of mixers: batch and continuous. Batch mixers handle one batch at a time, thoroughly combining materials before discharging and receiving the next batch. In contrast, continuous mixers receive a steady flow of ingredients, mixing them consistently and discharging without interruption. Within batch mixers, tilting drum mixers mix with internal...
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Mixing Time01:19

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The concept of mixing time is significant in producing a uniform concrete mix with the required strength. The mixing period starts once all components are in the mixer. Initially, the mixer is charged with 10% of the water, followed by the consistent addition of solids and then 80% of the water. The remaining water is added later, within the first quarter of the mixing period. The minimum mixing time varies according to the mixer's capacity; for example, mixers with up to 1 cubic yard...
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Ready-mixed concrete, also known as pre-mixed concrete, is prepared in a centralized plant and then transported in trucks to construction sites where it is ready for placement. This type of concrete is categorized into central-mixed, truck-mixed (or transit-mixed), and shrink-mixed. Central-mixed concrete is entirely prepared at a plant and moved to the site in agitator trucks that rotate at a speed of 2 to 6 rpm. Truck-mixed concrete, on the other hand, has the ingredients batched at the plant...
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Design Example: Vintage Mixing Console01:17

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Interspecific interactions in a methane-utilizing mixed culture.

S C Lamb1, J C Garver1

  • 1Department of Biochemistry University of Wisconsin, Madison, Wisconsin 53706.

Biotechnology and Bioengineering
|January 19, 2018
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Summary

This study models interspecific interactions in methane-utilizing bacterial cultures. A mathematical model based on pure culture data accurately predicts mixed culture behavior in continuous systems.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemical Engineering

Background:

  • Mixed microbial cultures are vital in biotechnology.
  • Understanding interspecific interactions is key to optimizing culture performance.
  • Methane-utilizing bacteria play roles in carbon cycling and bioprocessing.

Purpose of the Study:

  • To investigate interspecific interactions in a two-member methane-utilizing bacterial culture.
  • To develop and validate a mathematical model for predicting mixed culture dynamics.
  • To elucidate the role of nutritional requirements and substrate ranges in microbial interactions.

Main Methods:

  • Continuous culture experiments were performed with isolated pure cultures and a mixed culture.
  • Nutritional requirements and substrate ranges of pure cultures were determined.
  • Product formation kinetics were measured.
  • A mathematical model was derived using material balance equations for a chemostat.
  • Model predictions were compared with experimental steady-state data.

Main Results:

  • A mechanism for interspecific interactions was proposed based on pure culture data.
  • The derived mathematical model accurately predicted the steady-state behavior of the two-member mixed culture.
  • Product formation kinetics were quantified for key intermediates.
  • The study provides insights into interactions in related methanol- and methane-utilizing mixed cultures.

Conclusions:

  • Interspecific interactions in methane-utilizing mixed cultures can be modeled effectively.
  • Pure culture characteristics are predictive of mixed culture performance.
  • This modeling approach can aid in the design and optimization of biotechnological processes using mixed microbial cultures.