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Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12

Chia-Wen Lo1, Chih-Sheng Chen2,3,4, Ying-Chi Chen5

  • 1School of Chinese Medicine, China Medical University, Taichung, Taiwan.

Molecular Nutrition & Food Research
|January 19, 2018
PubMed
Abstract

Insights

Allyl isothiocyanate (AITC) inhibits adipocyte differentiation by reducing galectin-12 levels. This compound also demonstrates anti-obesity effects in high-fat diet-induced obesity models.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Metabolic Research

Background:

  • Obesity is a complex metabolic disorder linked to adipocyte dysfunction.
  • Galectin-12 plays a role in adipogenesis and obesity.
  • Allyl isothiocyanate (AITC) is a natural compound with potential therapeutic properties.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying AITC's anti-adipogenic effects.
  • To investigate AITC's impact on galectin-12 expression.
  • To evaluate AITC's efficacy in a high-fat diet (HFD)-induced obesity mouse model.

Main Methods:

  • 3T3-L1 cells were used to assess AITC's effects on adipocyte differentiation.
  • Key adipogenic transcription factors and signaling pathways (PKB/Akt/CREB) were analyzed.
  • Mice were fed a HFD and treated with AITC to evaluate its in vivo anti-obesity effects.

Main Results:

  • AITC dose-dependently inhibited lipid droplet accumulation in 3T3-L1 cells.
  • AITC suppressed adipocyte differentiation by downregulating galectin-12 expression and key transcription factors.
  • AITC modulated adipokine expression (leptin, resistin) and the PKB/Akt/CREB pathway.
  • In HFD-fed mice, AITC reduced body weight, liver lipid accumulation, and adipocyte size.

Conclusions:

  • AITC effectively inhibits adipocyte differentiation, partly through suppressing galectin-12.
  • AITC exhibits significant anti-obesity effects in a preclinical model of HFD-induced obesity.
Keywords:
3T3-L1Allyl isothiocyanateadipocytesgalectin-12obesity

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